Evaluación de diversidad genética molecular de especies de cítricos cultivadas en Irán reveladas por marcadores moleculares SSR, ISSR y CAPS

  • Ata Allah Sharafi University of Guilan
  • Asad Asadi Abkenar Agricultural Biotechnology Research Institute of Iran
  • Ali Sharafi Zanjan University of Medical Sciences
Palabras clave: Marcadores CAPS, Citrus, Diversidad Genética, Marcadores ISSR, Marcadores SSR


En este estudio, se analizó la diversidad genética en 19 cultivares de cítricos mediante la repetición de secuencia simple (SSR), la repetición de secuencia inter-simple (ISSR) y los marcadores de secuencia polimórfica amplificada segmentada (CAPS). Se utilizaron nueve cebadores para SSR, nueve cebadores ISSR y dos cebadores para CAPS para la puntuación del alelo. Se analizaron una región de ADN de cloroplasto (rbcL-ORF106) y una región de ADN mitocondrial (18S-5S) usando marcador de secuencia polimórfica amplificada escindida (CAPS) en 19 accesiones de cítricos cultivadas en Irán. En total, se detectaron 45 genes SSR y 131 ISSR polimórficos alelos y organelos del genoma del árbol. El análisis de conglomerados de los datos SSR y ISSR se realizó utilizando el método UPGMA y se basó en el coeficiente de Jaccard. El resultado de esta investigación mostró que los cebadores SSR e ISSR eran altamente informativos y eficientes para detectar la variabilidad genética y las relaciones de las accesiones de los cítricos. Y el análisis de marcadores de CAPS Los resultados mostraron que Bakraee y uno de tipo off cal mexicana tenían un patrón de bandas similar al Clementine Mandarin, mientras que Pummelo se consideraba como padre materno de otros genotipos estudiados Citron considerado como padre padre mostró un patrón de bandas definido entre 19 genotipos estudiados que confirmó Citoplasma herencia de orgánulos celulares de la madre.  


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Cómo citar
Sharafi, A. A., Abkenar, A. A., & Sharafi, A. (2017). Evaluación de diversidad genética molecular de especies de cítricos cultivadas en Irán reveladas por marcadores moleculares SSR, ISSR y CAPS. Journal of Science and Research: Revista Ciencia E Investigación, 2(8), 22-27. https://doi.org/https://doi.org/10.26910/issn.2528-8083vol2iss8.2017pp22-27
Artículo de Investigación