Evaluación de diversidad genética molecular de especies de cítricos cultivadas en Irán reveladas por marcadores moleculares SSR, ISSR y CAPS


  • Ata Allah Sharafi University of Guilan
  • Asad Asadi Abkenar Agricultural Biotechnology Research Institute of Iran
  • Ali Sharafi Zanjan University of Medical Sciences



Palabras clave:

Marcadores CAPS, Citrus, Diversidad Genética, Marcadores ISSR, Marcadores SSR


En este estudio, se analizó la diversidad genética en 19 cultivares de cítricos mediante la repetición de secuencia simple (SSR), la repetición de secuencia inter-simple (ISSR) y los marcadores de secuencia polimórfica amplificada segmentada (CAPS). Se utilizaron nueve cebadores para SSR, nueve cebadores ISSR y dos cebadores para CAPS para la puntuación del alelo. Se analizaron una región de ADN de cloroplasto (rbcL-ORF106) y una región de ADN mitocondrial (18S-5S) usando marcador de secuencia polimórfica amplificada escindida (CAPS) en 19 accesiones de cítricos cultivadas en Irán. En total, se detectaron 45 genes SSR y 131 ISSR polimórficos alelos y organelos del genoma del árbol. El análisis de conglomerados de los datos SSR y ISSR se realizó utilizando el método UPGMA y se basó en el coeficiente de Jaccard. El resultado de esta investigación mostró que los cebadores SSR e ISSR eran altamente informativos y eficientes para detectar la variabilidad genética y las relaciones de las accesiones de los cítricos. Y el análisis de marcadores de CAPS Los resultados mostraron que Bakraee y uno de tipo off cal mexicana tenían un patrón de bandas similar al Clementine Mandarin, mientras que Pummelo se consideraba como padre materno de otros genotipos estudiados Citron considerado como padre padre mostró un patrón de bandas definido entre 19 genotipos estudiados que confirmó Citoplasma herencia de orgánulos celulares de la madre.



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Ahmad R, Struss D, Southwick SM (2003) Development and Characterization of Microsatellite Markers in Citrus.AmerSocHortSci 128(4): 584-590

Asadi Abkenar A., Isshiki S., Tashiro Y. (2004a). Maternal inheritance of chloroplast DNA in intergeneric sexual hybrids of "true citrus fruit trees" revealed by PCR-RFLP analysis. Journal of Horticultural Science and Biotechnology, 79: 360-363.

Asadi Abkenar A., Isshiki S., Tashiro, Y. (2004b). Phylogenetic relationships in the "true citrus fruit trees" revealed by PCR-RFLP analysis of cpDNA. Scientia Horticulturae, 102: 233-242.

Awasthi A.K, Kar P.K, Srivastava P.P, Rawat N, Vijayan K, Pradeep, A.R, RajeUrs S (2008) Molecular evaluation of bivoltine, polyvoltine and mutant silkworm (Bombyxmori L.) with RAPD, ISSR and RFLP-STS markers. Indian Journal of biotechnology, 7: 188-194

Bayer R. J., Mabberley D. J., Morton C., Miller C. H., Sharma I. K., Pfeil B. E., Rich S., Hitchcock R., Sykes S. (2009). A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. American Journal of Botany, 96: 668–685.

Biswas MK, Xu Q, Deng X (2010) Utility of RAPD, ISSR, IRAP and REMAP markers for the genetic analysis of Citrus spp. SciHort 124: 254-261

De Riek J, Calsyn E, Everaert I, Van Bockstaele E, De Loose M (2001) AFLP based alternatives for the assessment of Distinctness, Uniformity and Stability of sugar beet varieties. TheorAppl Genet. 103:1254–1265

Dorji K., Yapwattanaphun C. (2015). Assessment of the genetic variability amongst mandarin (Citrus reticulata Blanco) accessions in Bhutan using AFLP markers. BMC Genetics, Advance online publication. doi: 10.1186/s12863-015-0198-8.

Faghihi MM, Bagheri AN, Bahrami HR., Hasanzadeh H, Rezazadeh R, Siampour M, Samavi S, Salehi M, Izadpanah K (2011). Witches’-broom disease of lime affects seed germination and seedling growth but is not seed transmissible. Plant Dis 95: 419-422

Fang DQ, Roose ML (1997) Identification of closely related citrus cultivars with inter–simple sequence repeat markers.TheorAppl Genet 95: 408–417

Froelicher Y., Mouhaya W., Bassene J-B, Constantino G., Kamir M., Luro F., Morillon R., Ollitrault P. (2011). New universal mitochondrial PCR markers reveal new information on maternal citrus phylogeny. Tree Genetics and Genomes, 7: 49-61.

Fujii H., Ohta S., Nonaka K., Katayose Y., Matsumoto T., Endo T., Yoshioka T., Omura M., Shimada T. (2016).
Parental diagnosis of satsuma mandarin (Citrus unshiu Marc.) revealed by nuclear and cytoplasmic markers. Breeding Science, 66: 683–691.

Golein B, Bigonah M, Azadvar M, Golmohammadi M (2012) Analysis of genetic relationship between ‘Bakraee’ (Citrus sp.) and some known Citrus genotypes through SSR and PCR-RFLP markers. SciHortic 148: 147-153

Green R. M., Vardi A., Galun E. (1986). The plastome of Citrus. Physical map, variation among Citrus cultivars and species and comparison with related genera. Theoretical and Applied Genetics, 72: 170-177.

Kijas JMH, Thomas MR, Fowler JCS, Roose ML (1997) Integration of trinucleotide microsatellites into a linkage map of Citrus. Theor Appl Genet 94: 701-706

Krueger RR, Roose ML (2003) Use of molecular markers in the management of Citrus germplasm resources. J AmerSocHortSci 128: 827-837

Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27: 209–220

Marak CK, Laskar MA (2010) Analysis of phonetic relationship between citrus indica Tanaka and a few commercially important citrus species by ISSR markers. SciHort 124: 345–348

Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325

Nicolosi E., Deng Z. N., Gentile A., La Malfa S., Continella G., Tribulato E. (2000). Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theoretical and Applied Genetics, 100: 1155-1166.

Ninomiya T., Shimada T., Endo T., Nonaka K., Omura M., Fujii, H. (2015). Development of citrus cultivar identification by CAPS markers and parentage analysis. Horticultural Research (Japan), 14: 127–133.

Nonaka K., Fujii H., Kita M., Shimada T., Endo T., Yoshioka T., Omura, M. (2017). Identification and parentage analysis of citrus cultivars developed in Japan by CAPS markers. The Horticulture Journal, 86: 208-221.

Novelli VM, Cristofani M, Machado MA (2000) Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck). Acta Hort 535: 47–9

Oliveira AC, Garcia AN, Cristofani M, Machado MA (2002) Identification of citrus hybrids through the combination of leaf apex morphology and SSR markers. Euphytica 128: 397-403

Palmer J. D., Jansen R. K., Michaels H. J., Chase M. W., Manhart, J. R. (1988). Chloroplast DNA variation and plant phylogeny. Annals of the Missouri Botanical Garden, 75: 1180-1206.

Pang XM, Hu CG, Deng XX (2003) Phylogenetic relationships among citrus and its relatives as revealed by SSR markers. Acta Genetica Sinica 30: 81–87

Peakall R, Smouse PE (2012) GenALEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update. Bioinformatics. Doi: 10.1093/bioinformatics/bts460

Ruiz C, Breto MP, Asins MJ (2000) A quick methodology to identify sexual seedlings in citrus breeding programs using SSR markers. Euphytica 112: 89–94

Rohlf FJ (2000) NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System. version 2.11. New York, Exeter, Setauket

Sanker AA, Moore GA (2001) Evaluation of inter–simple sequence repeat analysis for mapping in citrus and extension of the genetic linkage map. TheorAppl Genet 102: 206–214

Shahsavar AR, Izadpanah K, Tafazoli E, Sayed Tabatabaei BE (2007) Characterization of citrus germplasm including unknown variants by inter-simple sequence repeat (ISSR) markers. SciHortic 112: 310-314

Sharafi AA, Asadi Abkenar A, Sharafi A, Masaeli M (2016) Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers. Physiol Mol Biol Plants DOI 10.1007/s12298-016-0336-4

Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O, Gulsen O. (2009) Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). SciHortic 121: 306-312

Uzun A, Gulsen O, Yesiloglu T, Aka-Kacar Y, Tuzcu O (2010) Distinguishing grapefruit and pummel accessions using ISSR markers. Czech J Genet Plant Breed 46: 170-177

Watanabe M, Yamamoto T, Ohara M, Nishitani C, Yahata S (2008) Cultivar differentiation identified by SSR markers and the application for polyploidy loquat plants. J Japan Soc Hort Sci 77: 388-394

Yamamoto M., Kobayashi S., Nakamura Y., Yamada Y. (1993). Phylogenetic relationships of citrus revealed by analysis of mitochondrial and chloroplast DNA. Japanese Journal of Breeding, 43: 355-365.

Yamamoto M., Tsuchimochi Y., Ninomiya T., Koga T., Kitajima A., Yamasaki A., Inafuku-Teramoto S., Yang Xu, Yang Xi, Zhong G., Nasir N., Kubo T., Tominaga Sh. (2013). Diversity of Chloroplast DNA in Various Mandarins (Citrus spp.) and Other Citrus Demonstrated by CAPS Analysis. Journal of Japanese Society for Horticultural Science, 82: 106–113.

Zhen-hua L., Zhi-qin Zh., Rang-jin X. (2011). Molecular phylogeny of the True Citrus Fruit Trees Group (Aurantioideae, Rutaceae) as inferred from chloroplast DNA sequence. Agricultural Sciences in China, 10: 49-57.



Resumen 784

Cómo citar

Sharafi, A. A., Abkenar, A. A., & Sharafi, A. (2017). Evaluación de diversidad genética molecular de especies de cítricos cultivadas en Irán reveladas por marcadores moleculares SSR, ISSR y CAPS. Journal of Science and Research, 2(8), 22–27. https://doi.org/10.26910/issn.2528-8083vol2iss8.2017pp22-27



Artículo de Investigación